BREAKING — Tune Therapeutics closes $175M+ Series B to edit the genome's volume, not its sequence TUNE-401 enters first-in-human trials for chronic hepatitis B in New Zealand & Hong Kong Backed by NEA · Yosemite · Regeneron Ventures · Hevolution Foundation Spun out of Duke's Gersbach Lab — clinic-bound in ~5 years John McHutchison, MD (ex-Gilead) named CEO & Chairman BREAKING — Tune Therapeutics closes $175M+ Series B to edit the genome's volume, not its sequence TUNE-401 enters first-in-human trials for chronic hepatitis B in New Zealand & Hong Kong Backed by NEA · Yosemite · Regeneron Ventures · Hevolution Foundation Spun out of Duke's Gersbach Lab — clinic-bound in ~5 years John McHutchison, MD (ex-Gilead) named CEO & Chairman
Genetic Medicine · Durham & Seattle

Tune Therapeutics

The biotech that treats the genome like a mixing board - riding gene activity up and down without ever cutting the DNA.

Founded 2021 Epigenome editing ~68 employees $175M Series B Clinical stage
Tune Therapeutics logo
The mark. A square of quiet confidence from a company whose whole pitch is restraint - turn the gene down, don't tear it out.
The Dispatch

A lab in Durham is learning to whisper to your genes

Walk into the conversation around Tune Therapeutics in 2026 and you will not hear the usual gene-editing bravado about cutting, deleting, rewriting. You will hear something stranger and quieter. Tune does not want to change what your DNA says. It wants to change how loudly it is read. The company calls this epigenetic editing, and for the first time it is happening inside actual patients - people with chronic hepatitis B, sitting in clinics in New Zealand and Hong Kong, receiving a drug designed to turn a disease gene down and persuade it to stay down.

That is the whole heresy in one sentence. Most of biotech's celebrated tools - CRISPR and its cousins - are scissors. They make a permanent cut. Tune built a fader instead: a way to dial a gene's expression up, down, or off using the cell's own regulatory machinery, the epigenome, while leaving the underlying genetic code untouched. It is reversible. It is programmable. And it is, in the most literal sense, a tune.

Mastering the Epigenome - Transforming Human Healthspan.
— Tune Therapeutics, company mission
By The Numbers
$175M
Series B (Jan 2025)
2021
Founded
~68
Employees
2
HQ cities: Durham & Seattle
The Mechanism

Scissors cut. Tune turns the dial.

The genome is hardware. The epigenome is the software that decides which parts run, and how hard. Tune's TEMPO platform writes to that software.

Classic gene editing

Cut the code
  • Permanently alters DNA sequence
  • Risk of off-target cuts
  • One-way, irreversible
  • Hard to fine-tune the dose

Tune's epigenetic editing

Change the volume
  • DNA letters left untouched
  • Activate, silence or fine-tune a gene
  • Reversible and programmable
  • Multiple genes at once (multiplex)
TEMPO — riding the gene faders (illustrative)
silence
down
tune
up
activate
reset
// One platform, full range of motion. Schematic only - not clinical data.
The Pipeline

What Tune is actually building

Platform

TEMPO

The engine. A proprietary genetic-tuning system that selectively activates, silences, or fine-tunes specific genes by editing epigenetic marks rather than the DNA sequence - reversible and multiplexable by design.

Lead program · Clinical

TUNE-401

A first-in-class epigenetic silencer for chronic hepatitis B, a virus that affects an estimated 250M+ people. Designed to durably shut down viral gene expression. Now in first-in-human trials in New Zealand and Hong Kong.

Frontier

Healthspan & regeneration

Beyond HBV, Tune applies TEMPO to gene activation, cell reprogramming and age-related disease - the long bet that dialing gene activity is medicine's next platform, not just a one-disease trick.

The People

Built by the people who named the field

Tune spun out of Duke University's Gersbach Lab in 2021. Its bench is unusually decorated for a company this young.

CG

Charles Gersbach

Co-Founder · Duke professor

The platform traces back to his lab at Duke's Pratt School of Engineering, where he directs the Center for Advanced Genomic Technologies.

FU

Fyodor Urnov

Co-Founder · Scientific Advisory Board

A pioneer of genome engineering credited with helping coin the very term "genome editing," long before Tune existed.

AM

Akira Matsuno

Co-Founder · Chief Financial Officer

Helps steer the financial machinery behind a capital-hungry, clinical-stage genetic-medicine company across two cities.

JM

John McHutchison, MD

CEO & Chairman (2025)

Former CSO and Head of R&D at Gilead Sciences and ex-CEO of Assembly Biosciences, with deep experience in hepatitis drug development.

Going beyond the code.
— Tune Therapeutics tagline
The Money

$175 million, and a guest list to match

The January 2025 Series B was one of the largest single rounds the epigenetic-editing field has seen - and the investors say something about the ambition.

RoundAmountDateNotable backers
Series A~$60M2021NEA, Emerson Collective, Hatteras
Series B$175M+Jan 2025NEA, Yosemite, Regeneron Ventures, Hevolution Foundation

Note the names. Regeneron Ventures brings pharma credibility. Hevolution Foundation - a fund built specifically to extend healthy human lifespan - signals that Tune's pitch was never just about one virus. It was about the genome's volume knob as a general-purpose tool. (Figures from public reporting; amounts approximate.)

The Tape

Five years, lab to clinic

2021
Spun out of Duke's Gersbach Lab; Series A funds the TEMPO platform.
Nov 2024
Clearance to begin clinical trials of TUNE-401 for hepatitis B in New Zealand, later expanding to Hong Kong.
Jan 2025
Completes over $175M Series B led by NEA, Yosemite, Regeneron Ventures and Hevolution Foundation.
Mar 2025
Names John McHutchison, MD as CEO and Chairman, succeeding Matt Kane.
2026
Reports early Phase 1b/2a clinical data for its epigenetic silencer around the EASL liver-disease congress.
Why It Matters

Who this is for

Patients

Chronic disease

The near-term promise is for people living with chronic hepatitis B - a condition that today has no reliable cure, only suppression.

Scientists & partners

A new modality

Researchers and pharma teams get a tunable, reversible alternative to permanent gene editing - potentially safer for chronic dosing.

The long game

Healthspan

If gene expression can be dialed, the same logic could touch aging, regeneration and a wide class of "gene-dysregulation" diseases.

Watch & Read

See it in their own words

Footnotes

Five things worth knowing

The Last Word

Back in the clinic

Return to those patients in New Zealand and Hong Kong. A year ago, a chronic-hepatitis-B diagnosis meant a lifetime of management - drugs that suppress, but a virus that waits. The people now enrolled in Tune's trials are not testing another suppressant. They are testing the idea that you can walk up to a misbehaving gene, turn it down, and walk away while it stays quiet.

Whether TUNE-401 ultimately works is a question only the data will answer, and biotech is humble for good reason. But the bet underneath it is already changing the conversation: that medicine's next leap is not louder editing but better listening - finding the right gene, at the right volume, and leaving the rest of the code alone. The mixing board is plugged in. Tune is reaching for the fader.

Connect

Find Tune Therapeutics

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