# BioSqueeze Inc.

> BioSqueeze Inc. is a Butte, Montana company and the self-described world leader in commercial biomineralization. Its core technology pumps low-viscosity, self-diverting fluids carrying naturally occurring soil bacteria into oil and gas wells, where the microbes precipitate crystalline calcium carbonate (limestone) to permanently seal leakage pathways as small as one micron. The result is a gas-tight barrier that eliminates sustained casing pressure and stops fugitive methane emissions, helping operators meet well-integrity and environmental obligations across producing, plugged, and storage wells.

- **Founded:** 2021
- **Headquarters:** Butte, Montana, United States
- **Founders:** Mark Ranalli (President & CEO)
- **Team size:** ~44 employees
- **Products:** BioSqueeze (DPAS BioSqueeze), MacroSeal, MicroSeal, NanoSeal, Advanced Cement Imaging (ACI)
- **Notable:** Sealed its first oil and gas well in 2019, Completed 400+ successful well treatments across North America, Reported success rate exceeding 90%

## Products & services

- **BioSqueeze (DPAS BioSqueeze)** — Flagship biomineralization treatment that delivers soil bacteria via low-viscosity, self-diverting fluids to precipitate crystalline calcium carbonate and permanently seal leakage pathways, eliminating sustained casing/annular pressure.
- **MacroSeal** — A low-viscosity fluid system that rapidly crystallizes into an impermeable limestone barrier stronger than existing cement, for larger defects up to about 10 mm.
- **MicroSeal** — A self-diverting low-viscosity fluid that flows into channels, micro-annuli and pinholes and forms a gas-tight seal in existing barriers.
- **NanoSeal** — A sub-micron particle fluid system that penetrates the smallest aperture gaps - able to travel thousands of feet - to stop fluid and gas migration, safe for environmentally sensitive applications.
- **Advanced Cement Imaging (ACI)** — Diagnostic technology for analyzing cement bond and locating leakage pathways before and after treatment.
- **ARO Mitigation Strategies** — Asset retirement obligation / abandonment risk optimization services for operators plugging and abandoning wells.

## Achievements

- Sealed its first oil and gas well in 2019
- Completed 400+ successful well treatments across North America
- Reported success rate exceeding 90%
- Operations spanning nine U.S. states and two Canadian provinces
- Raised $11.475M total funding across Seed and Series A
- Awarded DARPA (2022) and U.S. Air Force (2023) Phase II SBIR grants
- Won the Chair's Stewardship Small Business Award at the 2025 IOGCC Annual Conference for the DPAS BioSqueeze
- Launched MacroSeal, MicroSeal and NanoSeal product lines in January 2025

## Latest updates

- **2025-01** — Unveiled three new products - MacroSeal, MicroSeal and NanoSeal - for restoring cement and barrier integrity.
- **2025** — Won the Chair's Stewardship Small Business Award at the IOGCC Annual Conference in Anchorage, AK for the DPAS BioSqueeze.
- **2023-09** — Awarded an Air Force Phase II SBIR grant and unveiled Advanced Cement Imaging (ACI) technology.
- **2023-02** — Closed $7.4M Series A led by Valo Ventures to accelerate North American expansion.

## Links

- Website: https://biosqueeze.com
- LinkedIn: http://www.linkedin.com/company/biosqueeze

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Published by YesPress — https://yespress.io
Last updated: 2026-06-16